OPTIMASI PRODUKSI ANTIBAKTERI CAIRAN KULTUR ISOLAT BAKTERI (ISOLAT Te234) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

  • Sandi Kurniawan Departemen Farmasi, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Magelang, Jalan Mayjend Bambang Soegeng km 5, Mertoyudan 56172, Kabupaten Magelang, Jawa Tengah
  • Alfian Syarifuddin Fakultas Ilmu Kesehatan, Universitas Muhammadiyah
  • Herma Fanani Agusta Fakultas Ilmu Kesehatan, Universitas Muhammadiyah
  • Missya Putri Kurnia Pradani Fakultas Ilmu Kesehatan, Universitas Muhammadiyah
Keywords: Staphlococcus aureus, antibacterial, escherichia coli, isolate Te234

Abstract

Antibiotics are metabolic products produced by certain microorganisms that can also damage other microorganisms. About 22,000 secondary metabolites in the form of antibiotics are produced by microbes, 70% are produced by actinomycetes, 20% by fungi, 7% Bacillus spp. and 1–2% by other bacteria. This research was conducted to determine the optimal time for bacteria to produce antibacterial which can inhibit the growth of Staphylococcus aureus and Escherichia coli. The method used is the well method with a diameter of 6.00 mm wellbore. The test results obtained, isolates of Te234 can produce optimal antibacterial which can inhibit the growth of Staphyloccus aureus bacteria on the 13th day with inhibition zone diameter of 16.00 ± 4.00mm against Escherichia coli bacteria on the 14th day with inhibitory zone diameter of 15, 33 ± 1.15mm so that it can be used as a determinant of the incubation time to produce antibacterial compounds. The TE 234 isolates are able to produce antibiotics that have the potential for the Staphylococcus aureus bacteria optimally on the 13th day, while on Escherichia coli on the 14th day

References

Cheng, M.-J., Tseng, M., Chen, I.-S., Liao, C.-C., & Yuan, G.-F. (2009). SECONDARY METABOLITES FROM THE CULTURE BROTH OF ACTINOMYCETE ACROCARPOSPORA SP. FIRDI 001 AND THEIR ANTIMICROBIAL ACTIVITY. Journal of the Chilean Chemical Society, 54(2). https://doi.org/10.4067/S0717-97072009000200024

Hamza, T. A., & Woldesenbet, F. (2017). Optimization of Culture Growth Parameters for Production of Protease from Bacteria, Isolated from Soil. 3(1), 1–10.

Khucharoenphaisan, K., Sripairoj, N., & Sinma, K. (2012). Isolation and Identification of Actinomycetes from Termite’s Gut Against Human Pathogen. Asian Journal of Animal and Veterinary Advances, 7(1), 68–73. https://doi.org/10.3923/ajava.2012.68.73

Owaga E.E, O. C. A. and C. K. N. E. (2009). Wasilatul Fadilla Hehehe. African Journal of Food, Agriculture, Nutrition and Development, 9(3), 1–8.

Ratnakomala, S., Apriliana, P., Fahrurrozi, & dan Wien Kusharyoto, P. (2016). Antibacterial activity of marine actinomycetes from Enggano Island. Jurnal Ilmu-ilmu Hayati, 15(3), 275–283.

Sulistyani, N., & Narwanti, I. (2015). Aktivitas Cairan Kultur Bakteri Penghasil Antibiotik (Isolat P301) terhadap Staphylococcus aureus ATCC 25923 dan Optimasi Waktu Produksi Metabolit Sekunder. 13, 6.

Sunaryanto, R. (2011). Isolasi, purifikasi, identifikasi, dan optimasi medium fermentasi antibiotik yang dihasilkan oleh aktinomisetes laut.

Supartono, S., Wijayati, N., Herlina, L., & Ratnaningsih, E. (2011). PRODUKSI ANTIBIOTIKA OLEH Bacillus subtilis M10 DALAM MEDIA UREA-SORBITOL. Reaktor, 13(3), 185. https://doi.org/10.14710/reaktor.13.3.185-193

Syarifuddin, A., Kamal, S., Yuliastuti, F., Pradani, M. P. K., & Septianingrum, N. M. A. N. (2019). Ekstraksi Dan Identifikasi Metabolit Sekunder Dari Isolat Al6 Serta Potensinya Sebagai Antibakteri Terhadap Escherichia coli. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 6(2), 210. https://doi.org/10.29122/jbbi.v6i2.3516

Syarifuddin, A., Sulistyani, N., & Kintoko, K. (2018). Activity of Antibiotic Bacterial Isolate Kp13 and Cell Leakage Analysis of Escherichia coli Bacteria. JURNAL ILMU KEFARMASIAN INDONESIA, 16(2), 137. https://doi.org/10.35814/jifi.v16i2.529

Wang, X., Huang, L., Kang, Z., Buchenauer, H., & Gao, X. (2010). Optimization of the Fermentation Process of Actinomycete Strain Hhs.015 T. Journal of Biomedicine and Biotechnology, 2010, 1–10. https://doi.org/10.1155/2010/141876

Warsi. N, S. (2018). Optimasi Waktu Produksi Metabolit Sekunder dan Skrining Aktivitas Antibakteri Isolat ActinomycetesRizosfer Tanaman Tin (Ficus carica). 7(1), 15–24. https://doi.org/10.29238/teknolabjournal.v7i1.120

Published
2020-10-27
How to Cite
Kurniawan, S., Syarifuddin, A., Agusta, H., & Pradani, M. (2020). OPTIMASI PRODUKSI ANTIBAKTERI CAIRAN KULTUR ISOLAT BAKTERI (ISOLAT Te234) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus. Jurnal Ilmiah Ibnu Sina, 5(2), 211-219. https://doi.org/10.36387/jiis.v5i2.449
Section
Artikel