PENGEMBANGAN SEDIAAN EMULGEL MENGANDUNG MINYAK BIJI ANGGUR (Vitis vinifera L.) DAN UJI AKTVITAS ANTILUKA TERHADAP TIKUS WISTAR JANTAN (Rattus norvegicus)

  • Sani Ega Priani Prodi Farmasi UNISBA, Bandung
  • Indah Ayu Lestari Prodi Farmasi UNISBA, Bandung
  • Fetri Lestari Prodi Farmasi UNISBA, Bandung
Keywords: Emulgel, Black seed oil, wound healing, required HLB

Abstract

Grape seed oil has wound healing activity since contains compounds such as flavonoids, polyphenols, saponins and linoleic acid. The purpose of this study is formulate emulgel containing grape seed oil with good physical characteristic and observed the wound healing activities of the oil and formulated emulgel. The research was begin with grape seed oil characterization and required HLB determination. The emulgel was made using combination Tween 80 and Span 80 as emulgator and carbomer as gelling agent with variation of oil concentration (10%, 20% and 30%). The formulation then subjected to physical evaluations including organoleptic, pH, spreadability, viscosity and rheology, sterility, and stability tests. Wound healing activity test of selected formula was done on seven groups of male Wistar rats. The emulgel containing grape seed oil with HLB value 8, has good physical, sterility, and stability characteristics. In vivo activity test showed that emulgel containing 20% and 30% grape seed oil have wound healing activity that statistically different compared with control (P<0.05) and the activity was not statistically different with marketed product (P>0.05).

References

Aisyah, A.N., Zulham, Nurul, A. Y., 2017, ‘Formulation of Emulgel Ethanol Extract of Mullberry (Morus alba L.) with Various Concentration of Span 80 and Tween 80’, Journal of Pharmaceutical and Medicinal Sciences, 2(2), 77-80.

Ansari, S. A., 2009, Handbook of Cosmetics Science and Technology. New York: Infoma Healthcare, 222.

Bayati, A.J., Duha F.E., 2015, ‘Histopathological Study and Surgery the Effect of Grape Seed Oil on Wound Healing in Rabbits’, International Journal of Science and Research. 4(6), 959-965.

Berdey, I.I., Voyt, O.I., 2016, ‘Rheological properties of emulgel formulations based on different gelling agent’, The Pharma Innovation Journal, 5(4): 76-79.

Departemen Kesehatan Republik Indonesia. (2015). Farmakope Indonesia, Edisi V, Direktorat Jenderal Pengawasan Obat dan Makanan, Jakarta, 859.

Febram, B., Ietje, W. Bambang, P., 2010, ‘Aktivitas Sediaan Salep Ekstrak Batang Pohon Pisang Ambon Dalam Proses Penyembuhan Luka Pada Mencit’, Majalah Obat Tradisional, 15(3), 121 – 137.

Garg, A., Anggarwal, D., Garg, S., Sigla, A. K., 2002, ‘Spreading of SemisolidFormulation, an update’, Pharmaceutical Technology, 9, 84-104.

Hait, S., Moulik, S., 2001, ‘Determination of Critical Micelle Concentration (CMC) of Nonionic Surfactants by Donor-Acceptor Interaction with Iodine and Correlation of CMC with Hydrophile-Lipophile Balance and Other Parameters of the Surfactants’. Journal of Surfactants and Detergents, 4, 303-309.

Haneefa, M, Abid, Shahima, H., Guru, P. M., Chandini, N., 2014, ‘Formulation and Evaluation of Herbal Emulgel of Pothos scandens Linn for Burn Wound Healing Activity’, J. Pharm. Sci. & Res. Vol. 6(2), 63-67.

Kusuma, R., A. Samba, Siva R., 2014, ‘Formulation, Development, and Evaluation of Indomethacin Emulgel Using Pregelatinized Starch from Ipomoea batata Tubers’, Asian Journal of Pharmaceutic, 9 (4), 43-48.

Lodhi, S., Abhay, K.I., 2013, ‘Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin induced diabetic rats’, Asian Pacific Journal of Tropical Medicine, 2013, 253-259.

Mohammad, M., 2004, ‘Optimization of Chlorphenesin Emulgel Formulation’, The AAPS Journal, 6 (3), 81-87.

Mohanty, A., Pal., A., Sahu, P.K., 2016, ‘Wound healing activity of Barringtonia acutangula fruit extract’, Asian J Pharm Clin Res, 9(2), 236-238

Panwar, A.S., N. Upadhyay, M. Bairagi, S. Gujar, G. N. Darwhekar, D. K. Jain, 2011, ‘Emulgel: A Review’, Asian Journal of Pharmacy and Life Science’, 1(3), 333-340.

Priani, S.E., Haniva H., Fitrianti D., 2014, ‘Development of sunscreen emulgel containing cinnamomum burmannii stem bark extract’, International Journal of Science and Research, 3(12), 2338-2339.

Priani, S.E., Haniva H., Fitrianti D., 2014, ‘Formulasi Sediaan Emulgel Antioksidan Mengandung Ekstrak Etanol Kulit Batang Kayu Manis (Cinnamomum Burmanni Nees Ex. BL.)’, Prosiding SNaPP: Sains, Teknologi, 4(1), 103-110.

Rai, N., I.P. Pandey, 2013, ‘Study of some physiochemical factors determining emulsion stability with mixed Emulsifiers’, J. Ind. Res. & Technology, 3(1), 12-16.

Saputri, R., Aristha, N.P., 2017, ‘Potensi Ekstrak Etanol Herba Lampasau Sebagau Penyembuh Luka Sayat Pada Tikus’, Jurnal Borneo Journal of Pharmascientech, 1(1), 57-65.

Schmidts, T., P. Schlupp, A. Gross, D. Doble, F. Runkel, 2012, ‘Required HLB Determination of Some Pharmaceutical Oils in Submicron Emulsions’, Journal of Dispersion Science and Technology, 33(6), 816-820.

Shahin, M., Seham. A. H., Mohammed, H., Nahed. M., 2011, ‘Novel Jojoba Oil-Based Emulsion Gel Formulations for Clotrimazole Delivery’, AAPS PharmSciTech., 12(1), 239–247.

Sulistyaningsih , Dolih G. , Rd. M. Bambang, Resmi M., 2017, ‘Ketoconazole Emulgel Formula Activity Test against Microsporum gypseum and Candida albicans’, J. Pharm. Sci. & Res. Vol. 9(12), 2458-2462.

Yan, H., Peng, K.J., Wang, Q.L., Gu, Z.Y., Lu, Y.Q., Zhao, J., Xu, F., Liu, Y.L., Tang, Y., Deng, F.M., Zhou, P., Jin, J.G., Wang, X.C. 2013, ‘Effect of pomegranate peel polyphenol gel on cutaneous wound healing in alloxan-induced diabetic rats’, Chin. Med. J. , 126(9):1700-1706.

Published
2019-03-31
How to Cite
Priani, S., Lestari, I., & Lestari, F. (2019). PENGEMBANGAN SEDIAAN EMULGEL MENGANDUNG MINYAK BIJI ANGGUR (Vitis vinifera L.) DAN UJI AKTVITAS ANTILUKA TERHADAP TIKUS WISTAR JANTAN (Rattus norvegicus). Jurnal Ilmiah Ibnu Sina, 4(1), 66 - 76. https://doi.org/10.36387/jiis.v4i1.233
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