IDENTIFIKASI DAN MOLECULAR DOCKING KOMPONEN UTAMA MINYAK KULIT BUAH JERUK NIPIS SEBAGAI AGEN ANTIKANKER

  • Indah Hairunisa Program Studi Farmasi, Fakultas Kesehatan dan Farmasi, Universitas Muhammadiyah Kalimantan Timur
  • Normaidah Normaidah Program Studi S-1 Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lambung Mangkurat
  • Sylvan Septian Ressandy Program Studi Farmasi, Fakultas Kesehatan dan Farmasi, Universitas Muhammadiyah Kalimantan Timur
  • Fhirda Azhari Program Studi Farmasi, Fakultas Kesehatan dan Farmasi, Universitas Muhammadiyah Kalimantan Timur
Keywords: Lime, cancer, in silico, oil

Abstract

Cancer is the highest cause of death in the world. This disease is known to have different regulations from other diseases. The development of drugs today is to target anticancer drugs on cancer regulation sites at Hallmark of Cancer. Lime (Citrus aurantifolia) is one type of Citrus (orange) which contains beneficial chemical compounds and active as anticancer agents. Lime peel is known to have bioactive content in the form of essential oils and flavonoids such as hesperidin and hesperitin. The content of this compound is closely related to anticancer activity. This study aims to identify the content of compounds in lime peel oil and the study of molecular docking silico in these compounds in protein that play a role in the regulation of cancer cells. Lime peel oil is obtained using the steam distillation method then analyzed using GC-MS to determine its constituent components to be analyzed in silico then using YASARA 10.1.8, MarvinSketch 15.4.20, and PLANTS software. The results showed that the main components of lime peel oil were d-limonen and beta-pinen with a percentage of 59.71% and 36.81%. In silico analysis shows that each of these compounds has an affinity greater than the native ligand on caspase-8 proteins (protein regulation of apoptosis). Based on these results it can be concluded, that the lime peel oil has the potential to be developed as an anticancer agent.

References

Adina AB, Handoko FF, Setyarini II, Septistyani EP, Riyanto S, Meiyanto,E.. 2008. Ekstrak etanolik kulit jeruk nipis (Citrus aurantifolia (cristm.) Swingle) meningkatkan sensitivitas sel mcf-7 terhadap Doxorubicin. Proceeding Kongres Ilmiah XVI Ikatan Sarjana Farmasi Indonesia.; 55-62.

Adindaputri, Z. Purwanti, N dan Wahyudi, i.A. 2013. Pengaruh Ekstrak Kulit Jeruk Nipis (Citrus Aurantifolia Swingle) Konsentrasi 10% Terhadap Aktivitas Enzim Glukosiltransferase Streptococcus mutans. Maj Ked Gi. Desember 2013; 20(2):126-131.

Astawan W, Kasih AL. 2008. Khasiat Warna-Warni Makanan. Jakarta: PT Gramedia Pustaka. H. 31,101.

Gonzalez-Angulo, A.M., Morales-Vasquez, F., dan Hortobagyi, G.N. 2007. Overview of resistance to systemic therapy in patients with breast cancer. Adv Exp Med Biol. 608:1-22.

Kemenkes RI. 2015. Pusat Data dan Informasi Kementrian Kesehatan RI, Stop Kanker. Kementrian Kesehatan RI Pusat Data dan Informasi. Jakarta Selatan.

Pathan, R.K., Gali, P.R., Pathan, P., Gowtham, T. Pasupuleti, S. 2012. In vitro Antimicrobial Activity of Citrus aurantifolia and its Phytochemical Screening. Asian Pacific Journal of Tropical Disease S328-S331.

Patil J, Jayaprakasha G, Chidambara Murthy K, Tichy S, Chetti M, Patil B. 2009. Apoptosis-mediated proliferation inhibition of human colon cancer cells by volatile principles of Citrus aurantifolia. Food Chem;114:1351–8.

Purnomo, Hari. 2011. Kimia Komputasi : Molecular Docking Plants, Penambatan Molekul Plants (Protein-Ligand-Ant-System), Ilmu Semut. Pustaka Pelajar : Yogyakarta.

Radji, Maksum. 2014. Mekanisme Aksi Molekular Antibiotik dan Kemoterapi. Penerbit Buku Kedokteran EGC. Yogyakarta

Simas, D.L. R., Amorim, S. H. B. M., Goulart, F. R. V., Alviano, C. S., Alviano, D. S., Silva, A. J. R. 2017. Citrus Species Essential Oils and Their Components can Inhibit or Stimulat Fungal Growth in Fruit. Industrial Crops and Products 98, 108-115.

Wahyudi, Tatang., Mulyawan, A.S., Kasipah, Cica., Prayudie, Untung dan Julaeha, Euis. 2017. Pembuatan Mikrokapsul Minyak Jeruk Nipis (Citrus aurantifolia) Untuk Aplikasi Pada Penyempurnaan Tekstil. Arena Tekstil Vol.32, No:1.

Published
2019-10-31
How to Cite
Hairunisa, I., Normaidah, N., Ressandy, S., & Azhari, F. (2019). IDENTIFIKASI DAN MOLECULAR DOCKING KOMPONEN UTAMA MINYAK KULIT BUAH JERUK NIPIS SEBAGAI AGEN ANTIKANKER. Jurnal Ilmiah Ibnu Sina, 4(2), 314-322. https://doi.org/10.36387/jiis.v4i2.338
Section
Artikel