UJI AKTIVITAS ANTIBAKTERI FORMULASI SALEP HIDROKARBON DAUN PEPAYA (Carica papaya L.) SEBAGAI SALAH SATU ALTERNATIF OBAT PENYEMBUH LUKA

  • Erna Prihandiwati Akademi Farmasi ISFI Banjarmasin
  • Anna Khumaira Sari Akademi Farmasi ISFI Banjarmasin
Keywords: Papaya Leaf, Ointment, Formulation, Antibacterial Test

Abstract

Papaya (Carica papaya L) is a plant that can live in various places in Indonesia and requires a relatively short time to grow. Papaya leaves contain karpain alkaloid, karikaksantin, violaksantin, papain, saponins, flavonoids, tannins, karposid and saponins. The compounds that have antibacterial properties are karpain alkaloids. Ointment is one of the pharmaceutical preparations that is suitable for wound healing drugs with papaya leaf extract. This ointment is able to treat infections caused by staphylococcus aureus with great effectiveness and small side effects when compared with the use of chemicals. This study aims to determine the most effective formulations of hydrocarbon base ointment with physical properties test. Papaya leaf extract was obtained by maceration method for five days with 96% ethanol. Macerate is then evaporated with water bath until it becomes a thick extract of papaya leaves. Papaya leaf extract is then formulated at formula 2 and then carried out an antibacterial test with extract concentrations of 5; 10; 20; 30 and 40%. This research is experimental research. The results showed that formula 2 was the most effective based on the physical properties test of the ointment. Antibacterial test results showed that the highest inhibition zone diameter was obtained in the ointment of papaya leaf extract concentration of 40% in the amount of 11.63 ± 0.671441 mm and the smallest in the concentration of 5% in the amount of 5.63 ± 0.550757.

References

A’yun, Q., Laily, A, N., 2015, Analisis Fitokimia Daun Pepaya (Carica papaya L.) di Balai Penelitian Tanaman Aneka Kacang dan Umbi, Kendalpayak, Malang, Seminar Nasional Konservasi dan Pemanfaatan Sumber daya Alam

Anggraini, Novia Norlyta, 2017. Uji Sifat Fisik Formulasi Sediaan Emulgel Ekstrak Etanol Daun Pepaya (Carica papaya L.) Dengan Berbagai Variasi Gelling Agent, Karya Tulis Ilmiah, Akademi Farmasi ISFI Banjarmasin, Banjarmasin

Anief, M. 2007. Farmasetika. Gadjah Mada University Press, Yogyakarta

Cita, yatnita parama. 2011. Bakteri salmonella typhi dan demam tifoid. Jurnal kesehatan masyarakat. Vol.6 No.1 , pp.42–46

Himedia. 2011. Himedia Technical Data: Plate Count Agar (Standard Methods Agar). http://himedialabs.com/TD/M091.pdf

Kumar A.R. 2013. Antimicrobial Sensitivity Pattern of Klebsiella pneumonia Isolated from Pus from Tertiarycar Hospital and Issues Related to the Rational Selection of Antimicrobials. Journal of Chemical and Pharmaceutical Research. 5(11): 326331.

Pelczar, Michael J dan Chan, E. C. S. 2010. Dasar-Dasar Mikrobiologi Jilid I,Jakarta: UI Press.

Pratiwi. 2008. Mikrobiologi Farmasi. Jakarta: Erlangga.

Prihandiwati, E dan Sari, A.K., 2019, Formulasi dan Uji Sifat Fisik Sediaan Salep Ekstrak Daun Pepaya (Carica papaya L) dengan Basis Hidrokarbon, Proceeding of Unism National Pharmacy Seminar

Radji, M. 2016. Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta : Penerbit Buku Kedokteran EGC

Rostinawati, T. 2009. Aktivitas Antibakteri Ekstrak Etanol Bunga Rosella (Hibiscus Sabdariffa L.) Terhadap Escherichia coli, Salmonella typhidan Staphylococcus aureus dengan Metode Difusi Agar. Penelitian Mandiri Fakultas Farmasi Universitas Padjadjara

Rosyiedi, Afif Fajri, 2011, Uji Sifat Fisik Dan Iritasi Sediaan Salep Ekstrak Etanolik Daun Pepaya (Carica papaya L.) Dengan Basis Hidrokarbon Dan Absorbsi, Skripsi, Universitas Sebelas Maret, Surakarta

Saraswati, F.N, 2015, UJi Aktivitas Antibakteri Ekstrak Etanol 96% Limbah Kulit Pisang Kepok Kuning (Musa balbisiana) Terhadap Bakteri Penyebab Jerawat (Staphylococcus epidermis, Staphylococcus aureus, dan Propionibacterium acne). Skripsi. UIN Syarif Hidayatullah Jakarta

Septiningsih, Erna., 2008. Efek Penyembuhan Luka Bakar Ekstrak Etanol 70% Daun Pepaya (Carica papaya L.) Dalam Sediaan Gel Pada Kulit Punggung Kelinci New Zealand. Universitas Surakarta. Surakarta

Susanty., Bachmid, Fairus., 2016, Perbandingan Metode Ekstraksi Maserasi dan Refluksi Terhadap Kadar Fenolik Dari Ekstrak Tongkol Jagung (Zea mays L), KONVERSI Vol.5 No.2, Universitas Muhammadiyah Jakarta, Jakarta

Sutton, S., 2011, Determination of Inoculum for Microbiological Testing, Journal of GXP Compliance, 15 (3), 49-53.

Syarifah, R, S., Mulyanti, D., Gadri, A., 2015, Formulasi Sediaan Masker Gel Peel-Off Ekstrak Daun Pepapa (Carica papaya L.) Sebagai Antijerawat dan Uji Aktivitasnya terhadap Bakteri Propionibacterium Acnes, Prosiding Penelitian SPeSIA UNISBA, 662-670.

Published
2019-10-31
How to Cite
Prihandiwati, E., & Sari, A. (2019). UJI AKTIVITAS ANTIBAKTERI FORMULASI SALEP HIDROKARBON DAUN PEPAYA (Carica papaya L.) SEBAGAI SALAH SATU ALTERNATIF OBAT PENYEMBUH LUKA. Jurnal Ilmiah Ibnu Sina, 4(2), 380-390. https://doi.org/10.36387/jiis.v4i2.348
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Artikel