EFEK ADAPTOGENIK EKSTRAK ETANOL KULIT BUAH MANGGIS (Garcinia mangostana L.) PADA MENCIT (Mus musculus) MENGGUNAKAN METODE SWIMMING ENDURANCE TEST
AbstractAdaptogenic activity was related to antioxidant activity which can reduce the number of free radicals, which in turn will reduce stress and improve body balance. Mangosteen rind (Garcinia mangostana L.) with a large antioxidant content promises to be Adaptogen. This study aims to examine the Adaptogenic effects of Ethanol Extract of mangosteen peel (Garcinia Mangostana L.) Using the Swimming Endurance Test (SET) in Mice. Simplicia of Mangosteen Fruit extracted by maceration using 96% ethanol, then tested its Adaptogen activity based on swimming endurance test. The group consisted of negative control (NaCMC 1%), positive control (Vit C. 100 mg / kg BB) and 3 groups treatment of ethanol extract of mangosteen peel ranging from 100, 200 and 400 mg / kg BB where all groups were given oral 1x1 oral administration for 7 days and on the 8th day, a SET test were performed. After that, data analysis was performed statistically. The results showed that the ethanol extract of mangosteen peel had the ability to be Adaptogenic where mice swimming in all groups given mangosteen peel extract was longer than the negative group with a significant difference (p <0.05). Ethanol extract of mangosteen peel showed a strong adaptogenic antistress effect in mice based on increased swimming time ability of mice.
A.Sudheer, Hari Prasad Sapkota, Nim Bahadur Dangi, Nabin Wagle, R.Hima Bindu, B.Sreedhar Naik, S.Nagarjuna, Ashish Adhikari. 2015. Evaluation of adaptogenic activity of methanolic extract of leaves of Tamarindus indica in rats and mice. Indo American Journal of Pharm Research.5:(06).
Chrousos, G.P. 2009. Stress and Disorders of the Stress System. Endocrinology. Vol. 5: 374-381.
Dungir S. G, dkk . 2012. Aktivitas Antioksidan Ekstrak Fenolik dari Kulit Buah Manggis (Garcinia mangostana L.) Jurnal Mipa Unsrat Online 1: (1) 11-15
Gupta, Vanita, et al. 2005. Anti-stress and adaptogenic activity of L-arginine supplementation. Evidence-based complementary and alternative medicine 2:1, 93-97.
Habbu, P.V., K.M. Mahadevan, P.V. Kulkarni, C. Daulatsingh, V.P. Veerapur, and R.A. Shastry. 2010. Adaptogenic and In Vitro Antioxidant Activity of Flavonoids and Other Fraction of Argyreia speciosa (Burm.f) Boj. In Acute and Chronic Stress Paradigms in Rodents. Indian Journal of Experimental Biology. Vol.48: 53- 60.
Kothiyal and Ratan, 2011. Antistress Effect of Fagopyrum esculentum in Rats subjected to Forced Swimming Endurance Test. Pharmacologyonline 3: 290-296
Samirana P.O dkk , 2015. Uji Aktivitas Adaptogenik Ekstrak Etanol Daun Bidara (Ziziphus mauritiana Auct. Non Lamk.) dengan Metode Swimming Endurance Test pada Mencit Galur Balb/C. Jurnal Farmasi Udayana. Vol. 4: No. 2 , 56-59
Sandhya, K. Desai, et al. 2011. Antistress activity of Boerhaavia diffusa root extract and a polyherbal formulation containing Boerhaavia diffusa using cold restraint stress model. Int J Pharm Pharm Sci. 3:1, 130-132.
Santos E.W, et al. 2016..Hematological and biochemical reference values for C57BL/6, Swiss Webster and BALB/c mice. Braz. J. Vet. Res. Anim. Sci., São Paulo, v.53: n. 2, p. 138-145
Panossian, A. And G. Wikman. 2010. ReviewEffects of Adaptogens on the Central Nervous System and theMolecular Mechanisms Associated with Their Stress Protective Activity. Pharmaceuticals. Vol.3: 188-224.
Pardon MC, Gould GG, Garcia A, Phillips L, Cook MC, Miller SA, Mason PA, Morilak DA. 2002. Stress reactivity of the brain noradrenergic system in three strains differing in their neuroendocrine and behavioral responses to stress: Implication for susceptibility to stress- related neuropsychiatric disorders. Neuroscience 115(1) : 229-242.
Puspitasari L, Swastini D.A, Arisanti C.I.S. 2013. Skrinig fitokimia ekstrak etanol 95% kulit buah manggis (Garcinia mangostana L.) Jurnal Farmasi Udayana. Vol 2 No. 3 hal 1-4
Vinod, S. P. And H. Shivakumar. 2012. A Current Status of Adaptogens: Natural Remedy to Stress. Asian Pacific Journal of Tropical Disease. S480-S490.
Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage, Alcohol Res Health, 2004, vol. 27 (pg. 277-284).